[摘要] 目的 探讨采用PCR和细胞培养方法比较流感样病例不同标本的流感病毒检出情况。方法 分析我中心2013年12月～2014年3月收集的流感指征患者193例作为研究对象，分别采集鼻拭子和咽拭子各1份，分别通过荧光PCR、细胞培养对流感病毒进行检测。 结果 鼻拭子对B亚型流感病毒敏感性较高，通过PCR和MDCK细胞接种中阳性率高于咽拭子(P<0.05)，另外A (H1N1)pdm09亚型在咽拭子中检出率较高，差异有统计学意义(P<0.05)。而在H3N2亚型检出率中二者无明显差异(P>0.05)。 结论 流感病毒监测过程中，根据当地流行主要流感病毒亚型来选择鼻拭子和咽拭子，对于提高流感病毒检出率具有很重要的意义。

    [关键词] 荧光PCR；细胞培养；流感病毒；检出

    [中图分类号] R511.7 [文献标识码] B [文章编号] 2095-0616(2015)15-147-03

    [Abstract] Objective To explore influenza virus detection of different specimen for influenza-like cases by polymerase chain reaction (PCR) and cell culture. Methods 193 patients with influenza indications who were admitted to our center from December 2013 to March 2014 were selected as research objects. One nose swab and one throat swab were respectively collected and influenza virus was respectively detected by fluorescent PCR and cell culture. Results Nose swab was more sensitive to B subtype influenza virus and positive rate of nose swab by PCR and Madin Darby canine kidney (MDCK) cell culture was higher than that of the throat swab(P<0.05). In addition ......